Research Articles
Evaluating the Efficiency of Gene Silencing Oligonucleotides: A Comprehensive Guide for Therapeutic Development
This article provides a critical evaluation of the efficiency of different gene silencing oligonucleotides, including ASOs, siRNAs, and miRNAs, for researchers and drug development professionals.
AI-Driven Nucleic Acid Design: Optimizing Sequences for Therapeutics and Functional Genomics
This article provides a comprehensive overview of modern nucleic acid sequence design and optimization, tailored for researchers and drug development professionals.
Spectrophotometry for DNA and RNA Analysis: A Complete Guide to Concentration, Purity, and Best Practices
This article provides a comprehensive guide for researchers and drug development professionals on using UV-Vis spectrophotometry for the quantification and purity assessment of DNA and RNA.
Strategies for Reducing Nuclease Degradation of Synthetic Oligonucleotides: From Backbone Modifications to Advanced Conjugates
This article provides a comprehensive overview of strategies to enhance the stability of synthetic oligonucleotides against nuclease degradation, a critical challenge in therapeutic and diagnostic applications.
Preserving Biomolecular Integrity: Advanced Methods for Long-Term RNA and Extracellular Vesicle Storage
This comprehensive guide details evidence-based protocols for preserving the structural and functional integrity of RNA and extracellular vesicles (EVs) during long-term storage.
Strategies for Improving Oligonucleotide Stability and Binding Affinity: From Chemical Design to Therapeutic Application
This article provides a comprehensive overview of the latest advancements and strategies for enhancing the stability and binding affinity of therapeutic oligonucleotides.
Mitigating RNAi Off-Target Effects: A Strategic Guide for Robust Experimental Design and Therapeutic Development
This article provides a comprehensive guide for researchers and drug development professionals on addressing the pervasive challenge of off-target effects in RNA interference (RNAi) experiments.
Eliminating DNA Contamination in RNA Preps: A Complete Guide for Accurate Gene Expression Analysis
This article provides a comprehensive guide for researchers and drug development professionals on addressing the critical challenge of genomic DNA contamination in RNA preparations.
The Complete Guide to PCR and RT-PCR Amplification Efficiency: From Foundational Principles to Advanced Troubleshooting
This comprehensive guide provides researchers, scientists, and drug development professionals with a systematic framework for understanding, optimizing, and validating PCR and RT-PCR amplification efficiency.
Preserving RNA Integrity: A Comprehensive Guide to Preventing Degradation During Isolation and Storage
This article provides researchers, scientists, and drug development professionals with a definitive guide to safeguarding RNA integrity from sample collection to long-term storage.