Research Articles
BGISEQ-500 vs Illumina HiSeq 2500/3000: A Comprehensive 2024 Comparative Guide for Whole Genome Sequencing
This article provides a detailed, evidence-based comparison of the BGISEQ-500 and Illumina HiSeq 2500/3000 platforms for whole-genome sequencing (WGS).
BEAMing Technology: A Comprehensive Guide to Ultra-Rare Variant Detection for Drug Development
This comprehensive guide explores BEAMing (Beads, Emulsion, Amplification, and Magnetics) technology, a powerful digital PCR-based method revolutionizing ultra-rare variant detection.
Geometric Efficiency in qPCR: A Comprehensive Guide for Robust Multi-Assay Analysis
This article provides a systematic framework for assessing geometric efficiency across multiplex and parallel qPCR assays, a critical but often overlooked parameter for data accuracy and reliability.
Housekeeping Genes in RT-PCR: A Comprehensive Guide to Efficiency Assessment, Best Practices, and Validation Strategies for Researchers
This article provides a systematic framework for researchers and drug development professionals to assess and optimize RT-PCR efficiency using housekeeping genes.
Clinical NGS Validation Blueprint: Analytical Standards for Robust Next-Generation Sequencing in Diagnostics and Drug Development
This article provides a comprehensive framework for the analytical validation of Next-Generation Sequencing (NGS) in clinical diagnostics and pharmaceutical research.
Amplicon vs. Hybrid-Capture NGS: A Complete Guide for Genomics Researchers in 2024
This comprehensive guide provides researchers, scientists, and drug development professionals with a detailed comparison of two dominant targeted next-generation sequencing (NGS) approaches: amplicon-based and hybridization capture methods.
Alu Elements and RNA Hyperediting: From Genomic Noise to Functional Significance in Disease and Drug Discovery
This article provides a comprehensive overview for researchers and drug development professionals on the critical intersection of Alu retrotransposons and adenosine-to-inosine (A-to-I) RNA editing in RNA-seq data analysis.
AlphaFold 3 Breakthrough: Transforming RNA-Ligand Complex Prediction for Drug Discovery
This article provides a comprehensive guide to AlphaFold 3 for researchers and drug development professionals seeking to model RNA-ligand complexes.
AlphaFold 3: The Ultimate Guide to Biomolecular Complex Prediction for Drug Discovery
This comprehensive guide examines AlphaFold 3, DeepMind's revolutionary AI system for predicting the 3D structures of biomolecular complexes, including proteins, DNA, RNA, ligands, and post-translational modifications.
Overcoming GC Bias in NGS: Strategies for Accurate Genomic and Clinical Research
GC bias, the uneven sequencing coverage of genomic regions based on their guanine-cytosine (GC) content, remains a critical challenge in next-generation sequencing (NGS), impacting data accuracy and reproducibility.