Research Articles
ADAR Editing vs. ASOs: A Comprehensive Comparison of Mechanisms, Efficacy, and Clinical Translation
This article provides a detailed comparative analysis of ADAR-mediated RNA editing and antisense oligonucleotide (ASO) technologies for therapeutic gene modulation.
RNA Editing Face-Off: ADAR vs. CRISPR-Cas13 - Mechanisms, Applications, and the Future of Therapeutic RNA Modification
This comprehensive review explores the two leading platforms for programmable RNA editing: endogenous ADAR enzyme-based systems and the prokaryotic-derived CRISPR-Cas13 machinery.
Decoding ADAR: A Comprehensive Guide to Enzyme Mechanism and Substrate Specificity for RNA Therapeutics
This article provides a detailed exploration of the Adenosine Deaminase Acting on RNA (ADAR) enzyme family, focusing on their catalytic mechanisms and the determinants of their substrate specificity.
Decoding A-to-I Editing: The Critical Role of Non-Coding RNAs and Alu Elements in Disease and Therapeutics
This article provides a comprehensive analysis of adenosine-to-inosine (A-to-I) RNA editing within non-coding RNAs and repetitive Alu elements, a critical yet underappreciated regulatory layer in human biology.
Comparing A-to-I RNA Editing Efficiency: Benchmarks for NGS, CRISPR, and Therapeutic Platforms
This article provides a comprehensive, up-to-date comparison of A-to-I RNA editing efficiency across major experimental and therapeutic platforms.
ADAR Enzymes and A-to-I RNA Editing: Mechanisms, Methods, and Therapeutic Applications in Modern Biomedicine
This comprehensive review explores the intricate mechanisms of adenosine-to-inosine (A-to-I) RNA editing catalyzed by ADAR enzymes, a crucial post-transcriptional regulatory process.
5′-(E)-Vinylphosphonate siRNA Modification: A Comprehensive Guide for Enhanced Stability and Potency
This article provides a detailed analysis of 5′-(E)-vinyl phosphonate (5′-E-VP) modification in siRNA therapeutics, tailored for researchers and drug development professionals.
2'-O-Methyl Modification of siRNA Guide Strands: Mechanisms, Protocols, and Best Practices for Off-Target Reduction
This comprehensive guide for researchers and drug development professionals explores the critical role of 2'-O-methyl (2'-OMe) modifications in siRNA guide strands for mitigating off-target effects.
V3-V4 vs. Full-Length 16S rRNA Sequencing: Choosing the Right Protocol for Microbiome Research & Drug Development
This article provides a comprehensive comparison of 16S rRNA gene sequencing protocols, focusing on the widely used V3-V4 hypervariable region approach versus emerging full-length sequencing.
DNA and RNA as Versatile Biomaterials: From Foundational Principles to Clinical Applications in Precision Medicine
This article provides a comprehensive exploration of DNA and RNA's expanding roles as engineered biomaterials beyond their biological functions.